Reprogramming somatic cells into stem cells
نویسندگان
چکیده
منابع مشابه
Differentiation of Somatic Unrestricted Cord Blood Stem Cells into Chondrocyte Cells
Purpose: To isolate and purify unrestricted somatic stem cells from human umbilical cord blood and evaluation of their differentiation into chondrocyte in vitro.Materials and Methods: In this study cells from human umbilical cord blood were isolated and plated in flask. Colonies were performed after one week. To determine the kind of cells, 100000 cells were analysed with flowcytometry. Twenty ...
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Factor-induced reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) is inefficient, complicating mechanistic studies. Here, we examined defined intermediate cell populations poised to becoming iPSCs by genome-wide analyses. We show that induced pluripotency elicits two transcriptional waves, which are driven by c-Myc/Klf4 (first wave) and Oct4/Sox2/Klf4 (second wave). Cell...
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Stem cells are the body’s ‘master’ cells, they are pluripotent cells, and in theory, they can potentially be differentiated to any tissue-type in the body. Until today, stem cells have been derived from the totipotent cells of the inner cell mass (ICM) of mouse and human blastocysts. The ability of a differentiated somatic cell to switch into a pluripotent cell has puzzled researchers for sever...
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The advent of human induced pluripotent stem cells (hiPSC) is revolutionizing many research fields including cell-replacement therapy, drug screening, physiopathology of specific diseases and more basic research such as embryonic development or diseases modeling. Despite the large number of reports on reprogramming methods, techniques in use remain globally inefficient. We present here a new op...
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Human embryonic stem cells (hESCs) are pluripotent and an invaluable cellular sources for in vitro disease modeling and regenerative medicine(1). It has been previously shown that human somatic cells can be reprogrammed to pluripotency by ectopic expression of four transcription factors (Oct4, Sox2, Klf4 and Myc) and become induced pluripotent stem cells (iPSCs)(2-4) . Like hESCs, human iPSCs a...
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ژورنال
عنوان ژورنال: Reproduction
سال: 2006
ISSN: 1470-1626,1741-7899
DOI: 10.1530/rep.1.01077